Dry virus vaccine against Newcastle disease Vladivak-La Sota, bottle of 100 doses
DESCRIPTION
Vladivak La Sota is a virus vaccine against Newcastle disease strain. The drug in appearance is a dry, porous white mass with a homogeneous and fine-grained structure, readily soluble in water without the formation of flakes and sediment.
COMPOSITION
Made from vaccinated extraembryonic fluid of SPF-chicken embryos infected with the Newcastle disease virus with the addition of skim milk as a stabilizer. One immunizing (nasal) dose contains at least 106.7 EID50 of the vaccine virus.
PHARMACOLOGICAL PROPERTIES
Birds vaccinated with the vaccine develop immunity to the causative agent of Newcastle disease 6 to 8 days after immunization, the intensity of which should be at least 3 months. The vaccine has no medicinal properties.
INDICATIONS
For the prevention of Newcastle disease of birds in prosperous and dysfunctional farms in various directions of cultivation.
DOSAGE AND APPLICATION
The vaccine is administered to poultry intranasally (ocularly), orally, by fine-dispersed (aerosol) and coarse-droplet spraying (spray method). A clinically healthy bird will lie down for vaccination, regardless of its age, breed, productivity (egg production) and molting period. 3 - 4 days before vaccination and within 5 days after it exclude giving the bird antibiotics, sulfa and nitrofuran preparations. The optimal timing of vaccination is determined by the results of RTGA or ELISA, examining at least 25 blood serum samples from birds in the poultry house (hall).
The first serological study is carried out in chickens at 7-10 days of age. The bird is vaccinated if, in 20% or more of blood serum samples, the antihemagglutin titer in RTGA is below 1: 8 or in ELISA - less than two minimum positive values ​​established by the instructions for use of the diagnostic kit used. If in 80% or more of blood serum samples the titer of maternal antibodies to the Newcastle disease virus in RTGA is 1: 8 and higher, and in ELISA it is more than two minimum values, then the bird is re-examined every 3 to 5 days and if the immunity is less than 80% the bird is vaccinated.
Revaccination of chickens is carried out if in more than 20% of blood serum samples the antibody titer in RTGA is less than 3 log2 (1: 8) or in ELISA - less than two minimum positive values ​​provided for in the instructions for use of the diagnostic kit used. When using the intranasal or intraocular method of administration, the vaccine is diluted at the rate of 1 immunizing dose of the drug, 0.1 ml of sterile 0.9% saline (pH 7.2 - 7.4). The diluted vaccine is instilled with an eye pipette into the nostril in a volume of 0.1 ml (2 drops each), while the other is closed with a finger, which achieves a deeper penetration of the vaccine into the nasal cavity. If the nasal opening is blocked, the same amount of vaccine can be applied to the conjunctiva of the eye. After intraocular injection, the bird's head is kept in a horizontal position for 1 - 2 seconds.
When using the enteral method (drinking with water), it is necessary to use fresh, clean, free from iron and chlorine ions, cooled to room temperature drinking water. The day before the application of the vaccine, determine the volume of water drunk by the bird for 1 - 1.5 hours and calculate its volume for the entire inoculated livestock. Birds are vaccinated at the rate of 10 immunizing (nasal) doses per head. The required number of doses of the vaccine, corresponding to the number of chickens in the inoculated batch, is diluted in a specified volume of water and poured into drinkers washed in advance without the use of disinfectants. The number of drinkers should ensure free access to the drug for all immunized livestock.
When immunizing meat-producing birds, the volume of water for dissolving the vaccine must be increased by 50%. To prevent the loss of activity of the vaccine strain, one of the following stabilizers is added to the water intended for vaccine dilution: 5% (by weight) skimmed milk powder, 25% (by volume) pasteurized milk (skim milk), 2.5% (by weight ) peptone GOST 13805-70, 2.5% enzymatic hydrolyzate of dry muscle (FGM-S) - TU 46-12-20-80. Before vaccination, the bird is kept without water and feed for 2 - 4 hours. Giving food and water to the bird is allowed 1.5 - 2 hours after vaccination.
When using the aerosol method, special generators are used that create a finely dispersed aerosol. With the aerosol method of immunization, the working dilution of the drug is determined by the formula: P. p. = (C x V x T x A) / D, where R. p. - working dilution of the vaccine; C is the concentration of the virus aerosol (in mg / l) in the room, the value of which is: 0.1 - in insufficiently pressurized poultry houses (cracks in windows and doors, small draft through the supply ventilation) and 0.2 - in satisfactorily pressurized rooms ( cracks in windows, insufficiently fitted windows, doors, hatches of ventilation shafts), 0.3 - in well-sealed conditions (carefully fitted windows, doors, hatches of ventilation shafts, no cracks). In poorly sealed poultry houses, aerosol vaccination is not carried out. V - pulmonary volume, l / min. It is calculated by the formula: V = 0, 78 x m - 16/1000, where 0.78 cm3 / min. per 1 g - the volume of respiration per 1 g of bird weight; m is the average weight of a bird (the average weight is determined by weighing 30 birds taken from separate places of the poultry houses), g; 16 - constant coefficient; 1000 - conversion of cm3 to liters. Further, according to 1 first formula: T - time of aerosol exposure to birds, which should not exceed 20 minutes. The exposure of immunization is counted after 2 - 3 minutes. since the start of aerosol generators. The exposure of immunization is counted after 2 - 3 minutes. since the start of aerosol generators. The exposure of immunization is counted after 2 - 3 minutes. since the start of aerosol generators.
On hot days, birds are immunized in the early morning hours at an air temperature in the house of no more than 20-22 В° C, and the exposure is reduced to 10 minutes, while reducing the dilution of the vaccine by 2 times; A - infectious activity of the vaccine virus (lg EID50 / cm3), which must be determined in advance before immunization and recalculated in lg EID50 / mg; D - the immunizing dose of the La Sota strain is 600 EID50 for chickens under 30 days old, for birds over one month old: 1000 - 1200 EID50. Example: the infectious activity of the virus is 9.0 lg EID50 / cm3 or 6.0 lg EID50 / mg (1.000.000 EID50 / mg). The aerosol concentration in the house is 0.1 mg / l. Exposure: 20 min. Pulmonary volume - 0.2 l / min. Virus dose for chickens over 30 days of age: 1000 EID50. R. p. = 0.1 x 0.2 x 20 x 1,000,000/1000 = 400, i.e. 1 cm3 of the vaccine virus must be diluted 1: 400. The total volume of lyophilized virus that must be taken to prepare a working dilution in a particular poultry house is determined based on its volume (cm3) and the working virus dilution.
Example: the volume of the house is 5000 m3, the working dilution of the virus is 1: 400. The consumption of the working virus dilution is 1 cm3 per 1 m3 of the poultry house. Thus, this house will require 5250 cm3 of the working virus dilution (5000 + 5% for the remainder in the aerosol generator). The amount of lyophilized virus will be 13.1 cm3 (5250: 400). Violations of the vaccination schedule should be avoided, as this can lead to a decrease in the effectiveness of immunization against Newcastle disease. If the next vaccine injection is missed, it is necessary to immunize as soon as possible.
SPECIAL INSTRUCTIONS
Products obtained from vaccinated birds are sold without any restrictions.
Protein components of the vaccine, especially when used in aerosol, have an individual allergenic effect, therefore, persons participating in the vaccination must use protective clothing (rubber boots, a dressing gown, trousers, a hat and rubber gloves), as well as goggles.
Persons with signs of respiratory, gastrointestinal, skin and allergic diseases are not allowed to participate in the vaccination.
Do not eat, drink or smoke during vaccination.
After work you should take a shower, wash your hands thoroughly with soap and change clothes.
If the vaccine comes into contact with the skin and mucous membranes, it is recommended to rinse them with plenty of tap water. In case of a vaccine spill, a 5% solution of chloramine or sodium hydroxide is poured over the floor or soil in this place.
CONTRAINDICATIONS
Vaccination of sick, suspicious and emaciated birds is not allowed.
Vaccination should not be carried out in conjunction with other live immunobiological preparations (except for spray vaccination in conjunction with the vaccine against infectious bronchitis of chickens), as well as immunization of chickens with other vaccines within 7 days after the next vaccination.
SIDE EFFECTS
In cases of an overdose of the vaccine used by the aerosol method, on the 4th - 5th day, young animals may experience malaise, shortness of breath, decreased appetite, and mortality may increase. These phenomena disappear by 10 - 12 days after vaccination. In adult birds, the post-vaccination reaction, as a rule, does not appear.
SHELF LIFE AND STORAGE
Store in a dry, dark place, out of reach of children and animals, in the manufacturer's packaging. Separated from food and feed at 2-8 В° C.
The shelf life is 12 months. Vials or ampoules with a vaccine without labels, with an expired shelf life, with a violation of integrity, with a changed color and consistency of the contents, with the presence of impurities, as well as vaccine residues that have not been used within 4 hours after opening, must be discarded and boiled for 30 minutes or treatment with 2% alkali solution, or 5% chloramine solution (1: 1) for 30 minutes, followed by disposal.
PACKAGING
Specifications
Bonus points
0
KolVUP
ten
Manufacturer
BiAgro
Temperature regime
from +2 to +8
Teaser
virus vaccine against Newcastle disease strain